This antibody is expected to recognize both reported isoforms (NP_071878.2, NP_001171275.1). Reported variants represent identical proteins: NP_071878.2, NP_001120823.1
纯化方法
Sirt3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Sirt3 antibody was raised against a amino acid synthetic peptide near the internal region of Sirt3 (mouse).
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 30 kDa band observed in mouse heart lysates (calculated MW of 28.8 kDa according to NP_071878.2). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 15 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.