This antibody is expected to recognize isoform 1 (NP_078808.3) only.
纯化方法
SPAG16 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SPAG16 antibody was raised against a 12 amino acid synthetic peptide near the Internal region of SPAG16.
SPAG16
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 55 kDa and 22 kDa in human testis lysates after 0.3 μg/mL antibody staining.8 kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.