This antibody is expected to recognize both reported isoforms (NP_005210.3, NP_001073280.1).
纯化方法
DIAPH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DIAPH1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of DIAPH1.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 140 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141 kDa according to human NP_005210.3 and 139 kDa according to mouse NP_031884.1). Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.