Western Blotting (WB), Immunoprecipitation (IP), ELISA
纯化方法
TRPC7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRPC7 antibody was raised against a 13 amino acid synthetic peptide near the internal region of TRPC7.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 20 kDa band in lysates of cell lines NIH3T3 and KELLY after 1 μg/mL antibody staining.6 kDa according to NP_065122.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.