RBM3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
RBM3 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of RBM3.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately17 kDa band observed in HEPG2 lysate (calculated MW of 17.2 kDa according to NP_006734.1). Recommended concentration: 0.01-0.03 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.