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- 抗原 See all BCKDHA 抗体
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This BCKDHA antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Antibody is purified by peptide affinity chromatography method.
- 免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCKDHA.
- Top Product
- Discover our top product BCKDHA Primary Antibody
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anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 268-407) antibody
BCKDHA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 347-445) antibodyBCKDHA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 362-390), (C-Term) antibodyBCKDHA 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20924 unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 1-445) antibodyBCKDHA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 71-120) antibodyBCKDHA 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, Cow, 马, 绵羊, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA) (AA 276-445) antibodyBCKDHA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- BCKDHA antibody can be used for detection of BCKDHA by ELISA at 1:312500. BCKDHA antibody can be used for detection of BCKDHA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- 注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store BCKDHA antibody at -20 °C.
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- 抗原
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
- 别名
- BCKDHA (BCKDHA 产品)
- 别名
- wu:fd20d04 antibody, zgc:110049 antibody, BCKDE1A antibody, MSU antibody, MSUD1 antibody, OVD1A antibody, BCKDA antibody, E1a antibody, 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody, branched chain keto acid dehydrogenase E1, alpha polypeptide antibody, 2-oxoisovalerate dehydrogenase alpha subunit (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A) antibody, branched chain ketoacid dehydrogenase E1, alpha polypeptide antibody, LOC704978 antibody, bckdha antibody, BCKDHA antibody, BckdhA antibody, Bckdha antibody
- 背景
- The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.
- 分子量
- 50 kDa
- 基因ID
- 593
- NCBI登录号
- NP_000700
- UniProt
- P12694
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