RFC5 抗体
Quick Overview for RFC5 抗体 (ABIN2462953)
抗原
See all RFC5 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC5.
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anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 1-340) antibody
RFC5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B1 unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) antibodyRFC5 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 170-200) antibodyRFC5 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal RB17319 unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (N-Term) antibodyRFC5 适用: 人, 犬, 豚鼠, 马, 猴, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (N-Term) antibodyRFC5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Cow, Saccharomyces cerevisiae, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 1-340) antibodyRFC5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Factor C (Activator 1) 5, 36.5kDa (RFC5) (AA 181-340) antibodyRFC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- RFC5 antibody can be used for detection of RFC5 by ELISA at 1:62500. RFC5 antibody can be used for detection of RFC5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC5 antibody at -20 °C.
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- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
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别名
- RFC5
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背景
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
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分子量
- 38 kDa
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基因ID
- 5985
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NCBI登录号
- NP_031396
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UniProt
- P40937
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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