POLR2I antibody can be used for detection of POLR2I by ELISA at 1:312500. POLR2I antibody can be used for detection of POLR2I by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR2I antibody at -20 °C.
抗原
POLR2I
(Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))
RPB9 antibody, hRPB14.5 antibody, GB18029 antibody, RPB14.5 antibody, 2810002B19Rik antibody, zgc:103515 antibody, RNA polymerase II subunit I antibody, DNA-directed RNA polymerase II subunit RPB9 antibody, DNA-directed RNA polymerase II 14.5 kDa polypeptide antibody, polymerase (RNA) II (DNA directed) polypeptide I antibody, polymerase (RNA) II subunit I L homeolog antibody, POLR2I antibody, LOC412547 antibody, LOC100282092 antibody, Polr2i antibody, polr2i antibody, polr2i.L antibody
背景
POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.