ID3 antibody can be used for detection of ID3 by ELISA at 1:1562500. ID3 antibody can be used for detection of ID3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store ID3 antibody at -20 °C.
抗原
ID3
(Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3))
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背景
ID3 is a member of the ID family. The members of the family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.