ALG1 antibody can be used for detection of ALG1 by ELISA at 1:62500. ALG1 antibody can be used for detection of ALG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALG1 antibody at -20 °C.
ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K).The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.