PDK1 antibody can be used for detection of PDK1 by ELISA at 1:1562500. PDK1 antibody can be used for detection of PDK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store PDK1 antibody at -20 °C.
抗原
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
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背景
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Please see the Entrez Gene record to access additional publications.