HBEGF 抗体 (EGF Like Domain) (Biotin)
(4 references)Quick Overview for HBEGF 抗体 (EGF Like Domain) (Biotin) (ABIN2451994)
抗原
See all HBEGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- EGF Like Domain
-
交叉反应 （详细）
- Does not react with mouse.
-
产品特性
- biotin/IgG = 7.5
-
纯化方法
- Purified
-
过滤
- Sterile filtered
-
免疫原
- Recombinant human HB-EGF ectodomain expressed in SF21 cell
-
亚型
- IgG1
-
-
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 51-150) antibody
HBEGF 适用: 人, 小鼠, 大鼠 ELISA, IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 21-160) antibodyHBEGF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (Bound), (EGF Like Domain), (Soluble) antibodyHBEGF 适用: 人 WB, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4G10 unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 63-148) antibodyHBEGF 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (AA 21-160) antibodyHBEGF 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) (N-Term) antibodyHBEGF 适用: 人, 小鼠, 大鼠, Cow, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) antibodyHBEGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) antibodyHBEGF 适用: 人 FACS 宿主: 兔 Chimeric unconjugated Fc fragment
anti-Heparin-Binding EGF-Like Growth Factor (HBEGF) antibodyKO Validated HBEGF 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
-
-
应用备注
-
1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS, 50 % glycerol
-
储存液
- Azide free
-
储存条件
- -20 °C/-80 °C
-
储存方法
- -20 C (For long term storage: -80 C)
-
-
-
: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).
: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).
: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).
: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).
-
: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).
-
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
-
别名
- HB-EGF
-
背景
- Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.
-
UniProt
- Q99075
-
途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
-
