ITK 抗体 (AA 1-26)
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Quick Overview for ITK 抗体 (AA 1-26) (ABIN2443927)
抗原
See all ITK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-26
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原理
- Itk (IL2-inducible T-cell kinase) Mouse Monoclonal Antibody (2F12)
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特异性
- A highly specific and sensitive antibody against Itk. The antibody recognizes both native and denatured Itk (amino acids 1-26). No detectable cross reactivity with other Itk family members such as Btk or Tec. S
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纯化方法
- Protein G purified
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100μL: WB 2 μg/mL. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- ITK (IL2-Inducible T-Cell Kinase (ITK))
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别名
- Itk (IL2-inducible T-cell kinase)
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背景
- IL2-inducible T-cell kinase (Itk) is located in cell membrane of T-cell lines and natural killer cell lines and plays a role in T-cell proliferation and differentiation. Defects in Itk are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1).
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UniProt
- Q08881
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway
抗原
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