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ITK 抗体 (Internal Region)

Verified ITK 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN1589997
发货至: 中国
  • 抗原 See all ITK 抗体
    ITK (IL2-Inducible T-Cell Kinase (ITK))
    抗原表位
    • 14
    • 10
    • 9
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 50
    • 19
    • 12
    • 5
    • 3
    人, 大鼠
    宿主
    • 63
    • 5
    • 1
    山羊
    克隆类型
    • 63
    • 6
    多克隆
    标记
    • 30
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This ITK antibody is un-conjugated
    应用范围
    • 42
    • 36
    • 14
    • 14
    • 11
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    原理
    ITK
    序列
    KERPEDRPAF SR
    交叉反应
    Cow, 人, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 70 kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4 kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    ITK (IL2-Inducible T-Cell Kinase (ITK))
    别名
    ITK (ITK 产品)
    别名
    Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody
    背景
    ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK
    基因ID
    3702
    NCBI登录号
    NP_005537
    途径
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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