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ITK 抗体 (C-Term)

Verified ITK 适用: 人 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184605
发货至: 中国
  • 抗原 See all ITK 抗体
    ITK (IL2-Inducible T-Cell Kinase (ITK))
    抗原表位
    • 14
    • 10
    • 9
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 50
    • 19
    • 12
    • 6
    • 3
    宿主
    • 63
    • 5
    • 1
    山羊
    克隆类型
    • 63
    • 6
    多克隆
    标记
    • 30
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This ITK antibody is un-conjugated
    应用范围
    • 42
    • 36
    • 14
    • 14
    • 12
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    原理
    ITK
    序列
    RLLRQLAEIA ESGL
    交叉反应
    人, 小鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.
    亚型
    IgG
    Top Product
    Discover our top product ITK Primary Antibody
  • 应用备注

    Western Blot: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:2000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentrat

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    ITK (IL2-Inducible T-Cell Kinase (ITK))
    别名
    ITK (ITK 产品)
    别名
    Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody
    背景
    ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258
    基因ID
    3702, 16428
    NCBI登录号
    NP_005537
    途径
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway
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