Collagen Type I 抗体
Quick Overview for Collagen Type I 抗体 (ABIN238355)
抗原
See all Collagen Type I (COL1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Bovine Collagen type I: 100 % . Bovine Collagen II, IX, XI: < 0.1 % Sheep Collagen type I: 50 % (IHC estimated) Bovine Fibronectin: < 0.1 % Human Collagen type I: < 2 % Chicken Collagen type I: < 0.1 % (Estimates except Sheep Collagen I from solid phase RIA at dilution 1/500). Even though cross-reactivity with human is low in RIA, the antibody has to be expected to be cross-reactiv to human Collagen type I as well as to pig Collagen type I in IHC.
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交叉反应 （详细）
- Species reactivity (tested):Bovine. Cross reacts with Sheep.
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纯化方法
- Affinity Chromatography
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免疫原
- Purified Collagen type I from Bovine skin
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), ICC, IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠, 哺乳动物, 鱼, Amphibian, Avian WB, IHC (fro), IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA, FLISA 宿主: 小鼠 Monoclonal 4F6 Biotin
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应用备注
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RIA. ELISA: 1/400 (OD > 500). Immunoblotting (Western blot). Immunofluorescence (1/80). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissues: 1/500-1/1000. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C overnight. Positive Control: Bovine skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL distilled water. For further dilution use appropriate antibody diluent.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without BSA and preservatives, None, None
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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别名
- Collagen Type I
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背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
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基因ID
- 282187
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NCBI登录号
- NP_001029211
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UniProt
- P02453
抗原
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