PPP1R13B 抗体
Quick Overview for PPP1R13B 抗体 (ABIN233830)
抗原
See all PPP1R13B 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-a18
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Supplier
- Rockland
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原理
- ASPP1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human ASPP1.
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产品特性
- Synonyms: rabbit anti-ASPP1 Antibody, ASPP-1, ASPP 1, p53BP2 like antibody, p85 antibody, PPP1R13B antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (AA 630-680) antibody
PPP1R13B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (AA 375-675) antibodyPPP1R13B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (AA 1-90) antibodyPPP1R13B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6H1 unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (Internal Region) antibodyPPP1R13B 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (AA 900-949) antibodyPPP1R13B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Bat, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) antibodyPPP1R13B 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) (Middle Region) antibodyPPP1R13B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to ASPP1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:2,500 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PPP1R13B (Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B))
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别名
- PPP1R13B
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背景
- Background: ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.
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基因ID
- 23368
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NCBI登录号
- NP_056131
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UniProt
- Q96KQ4
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途径
- p53 Pathway
抗原
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