JNK 抗体 (pThr183, Tyr185)
Quick Overview for JNK 抗体 (pThr183, Tyr185) (ABIN220911)
抗原
See all JNK (MAPK8) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr183, Tyr185
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特异性
- Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species cross-reactivity: mouse, monkey, hamster, bovine, rat and Drosophila. Species sequence homology: Xenopus.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IHC, IHC-P (1:75), IP (1:200), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:200. Immunohistochemistry (Paraffin): 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
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别名
- MAPK8 / JNK1 / JNK
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背景
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Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase -
基因ID
- 5599
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UniProt
- P45983
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途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
抗原
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