APEX1 抗体
Quick Overview for APEX1 抗体 (ABIN219055)
抗原
See all APEX1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human APE. Detects a band of ~37kD by Western Blot. Species cross-reactivity: mouse and rat.
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纯化方法
- Immunoaffinity purified
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免疫原
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Recombinant human APE.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ICC (1:100 - 1:200), IHC, IHC-Fr, IHC-P, WB (1:300 - 1:1000)
Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. Also suitable for use in Apoptosis assay (following TUNEL assay using Boehringer-Mannheim TUNEL assay kit). Western Blot: 1:300-1:1000 on HL60 and HeLa cells using ECL. Antibody may be used 1-4X in the binding solution depending on the cells/cell lines. Immunohistochemistry: 1:100-1:200 on frozen and paraffin sections from human and rat tissues. Immunocytochemistry: 1:100-1:200 on cytospin preparations of cells fixed in paraformaldehyde. Apoptosis (TUNEL) assay: perform antibody staining AFTER the. TUNEL assay. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS. Contains no preservatives.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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别名
- APEX1 / APE1
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背景
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Name/Gene ID: APEX1
Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1 -
基因ID
- 328
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UniProt
- P27695
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途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
抗原
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