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- 抗原 See all CD83 抗体
- CD83
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD83 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- The 4AHB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells.
- 克隆位点
- 4AHB15e
- 亚型
- IgG1
- Top Product
- Discover our top product CD83 Primary Antibody
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anti-CD83 (CD83) (AA 1-205) antibody
CD83 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3G10-1F4 unconjugated
anti-CD83 (CD83) antibodyCD83 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-CD83 (CD83) (AA 20-144) antibodyCD83 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 1B9B10 unconjugated
anti-CD83 (CD83) (AA 32-100) antibodyCD83 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD83 (CD83) (AA 24-205) antibodyCD83 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD83 (CD83) (AA 1-205) antibodyCD83 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F3 unconjugated
anti-CD83 (CD83) (Internal Region) antibodyCD83 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD83 (CD83) (AA 20-144) antibodyCD83 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD83 (CD83) (Internal Region) antibodyCD83 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD83
- 别名
- CD83 (CD83 产品)
- 别名
- BL11 antibody, HB15 antibody, CD83 molecule antibody, CD83 antigen antibody, Cd83 antibody, CD83 antibody
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