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- 抗原 See all CD56 (NCAM1) 抗体
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD56 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4AMEM188 reacts with CD56, a 175-220 kDa Neural Cell Adhesion Molecule (NCAM), expressed on 10-25 % of peripheral blood lymphocytes, including all CD16+ NK cells and approximately 5 % of CD3+ lymphocytes, referred to as NKT cells. It also is present at brain and neuromuscular junctions, certain LGL leukemias, small cell lung carcinomas, neuronally derived tumors, myeloma and myeloid leukemias. CD56 (NCAM) is involved in neuronal homotypic cell adhesion which is implicated in neural development, and in cell differentiation during embryogenesis.
- 克隆位点
- 4A123C3
- 亚型
- IgG1
- Top Product
- Discover our top product NCAM1 Primary Antibody
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anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 759-858) antibody
NCAM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibodyNCAM1 适用: 人, 小鼠, 猴 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5B1G11 unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (Internal Region) antibodyNCAM1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibodyNCAM1 适用: 人, 大鼠 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal NCAM1-795 unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (Internal Region) antibodyVerified NCAM1 适用: 人 FACS, WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibody (PerCP)NCAM1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-188 PerCP
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 568-708) antibodyNCAM1 适用: 人, 小鼠 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 1F6F10 unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 701-800) antibodyNCAM1 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 340-608) antibodyNCAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 829-863), (C-Term) antibodyNCAM1 适用: 人 FACS, WB 宿主: 兔 Polyclonal RB51136 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
- 别名
- CD56 (NCAM1 产品)
- 别名
- CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody
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