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- 抗原 See all CD48 抗体
- CD48
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD48 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4A156-4H9 reacts with blast-1, a 45 kDa GPI linked cell surface molecule. CD48 is expressed on peripheral blood lymphocytes, monocytes, or macrophages, but not on granulocytes and platelets nor on non-hematopoietic cells. CD48 binds to CD2 and plays a role as an accessory molecule in g/d T cell recognition and a/b T cell antigen recognition.* CD48 positive lymphocytes is decreased in patients with PNH (paroxysmal nocturnal haemoglobinuria)
- 克隆位点
- 4A156-4H9
- 亚型
- IgG1
- Top Product
- Discover our top product CD48 Primary Antibody
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anti-CD48 (CD48) antibody (Biotin)
CD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 Biotin
anti-CD48 (CD48) (AA 27-220) antibodyCD48 适用: 人 FACS, WB, ELISA, IF 宿主: 小鼠 Monoclonal 6F9H2 unconjugated
anti-CD48 (CD48) (AA 27-220) antibodyCD48 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM43 unconjugated Recombinant Antibody
anti-CD48 (CD48) antibodyCD48 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-102 unconjugated
anti-CD48 (CD48) antibody (FITC)CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 FITC
anti-CD48 (CD48) antibodyCD48 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-CD48 (CD48) (AA 1-243) antibodyCD48 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD48 (CD48) (AA 1-169) antibodyCD48 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B8-2C2 unconjugated
anti-CD48 (CD48) antibody (APC)CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 APC
anti-CD48 (CD48) antibody (PE)CD48 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-102 PE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD48
- 别名
- CD48 (CD48 产品)
- 别名
- AI449234 antibody, AW610730 antibody, BCM1 antibody, BLAST antibody, BLAST-1 antibody, BLAST1 antibody, Bcm-1 antibody, MEM-102 antibody, SLAMF2 antibody, Sgp-60 antibody, hCD48 antibody, mCD48 antibody, CD48 antigen antibody, Cd48 molecule antibody, CD48 molecule antibody, Cd48 antibody, CD48 antibody
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