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- 抗原 See all VCAM1 抗体
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VCAM1 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- CD106 is a 110 kDa single chain type I glycoprotein also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. It is expressed at high levels on the surface of cytokine-stimulated endothelium and at minimal levels on unstimulated endothelium.VCAM-1 serves as a ligand for the leucocyte integrins alpha 4 beta (CD49d/CD29 complex).A function of CD106 is to provide a costimulation signal in T cell activation. Reactivity with other species not tested.
- 克隆位点
- 4AP8B1
- 亚型
- IgG2a
- Top Product
- Discover our top product VCAM1 Primary Antibody
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anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 651-739) antibody
VCAM1 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibodyVCAM1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibodyVCAM1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H10 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 24-168) antibodyVCAM1 适用: 人, 大鼠, 猴 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6F12D7 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 109-318) antibodyVCAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-183) antibodyVCAM1 适用: 人, 猴 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6A11D8 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 300-400) antibodyVCAM1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 511-682) antibodyVCAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- CD106 (VCAM1 产品)
- 别名
- CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody
- 途径
- Carbohydrate Homeostasis
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