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- 抗原 See all CD58 抗体
- CD58
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD58 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4ATS2/9.1 reacts with 55-70 kDa CD58, lymphocyte function-associated antigen (LFA-3). It is expressed in hematipoietic and non-hematopoietic tissue including leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.CD58 mediates adhesion between killer and target cells, antigen presenting cells and T cells, thymocytes and thymic epithelial cells. It also provides co-stimulatory signal in immune response.
- 克隆位点
- 4ATS2-9-1-1-1
- 亚型
- IgG1
- Top Product
- Discover our top product CD58 Primary Antibody
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anti-CD58 (CD58) (AA 1-240) antibody
CD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) (AA 1-214) antibodyCD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 31-130) antibodyCD58 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 40-220) antibodyCD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD58 (CD58) (AA 1-241) antibodyCD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated
anti-CD58 (CD58) antibodyCD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated
anti-CD58 (CD58) antibody (Biotin)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin
anti-CD58 (CD58) antibody (PE)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE
anti-CD58 (CD58) antibody (APC)CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC
anti-CD58 (CD58) (AA 29-215) antibodyCD58 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD58
- 别名
- CD58 (CD58 产品)
- 别名
- LFA-3 antibody, LFA3 antibody, ag3 antibody, CD58 molecule antibody, CD58 antibody
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