CD13 抗体 (Biotin)
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- 抗原 See all CD13 (ANPEP) 抗体
- CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD13 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- 4AD43 reacts with CD13, 150 kDa aminopeptidase N (APN).
- 克隆位点
- 4AD43
- 亚型
- IgG2b
- Top Product
- Discover our top product ANPEP Primary Antibody
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anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 668-967) antibody
ANPEP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 344-444) antibodyANPEP 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 34-219) antibodyANPEP 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibodyANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal WM15 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibodyANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP, Func 宿主: 小鼠 Monoclonal WM15 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 781-967) antibodyANPEP 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 1C7D7 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (FITC)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 FITC
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (Biotin)ANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal WM15 Biotin
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (PE)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 PE
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (PerCP)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 PerCP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
- Repeat step 5.
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
- 别名
- CD13 (ANPEP 产品)
- 别名
- APN antibody, CD13 antibody, GP150 antibody, LAP1 antibody, P150 antibody, PEPN antibody, A2AP antibody, AAP antibody, ALPHA-2-PI antibody, API antibody, PLI antibody, AAT1 antibody, ALT1 antibody, GPT1 antibody, Gpt1 antibody, Apn antibody, pepN antibody, SC8E4A.13 antibody, 1300007J06Rik antibody, 2310022B03Rik antibody, ALT antibody, Gpt-1 antibody, AP-M antibody, AP-N antibody, Cd13 antibody, Apm antibody, KZP antibody, Lap1 antibody, rAPN antibody, APDE antibody, p150 antibody, anpep antibody, fb64c05 antibody, wu:fb64c05 antibody, zgc:136771 antibody, alanyl aminopeptidase, membrane antibody, serpin family F member 2 antibody, glutamic--pyruvic transaminase antibody, aminopeptidase N antibody, aminopeptidase antibody, aminopeptidase n antibody, glutamic pyruvic transaminase, soluble antibody, alanyl (membrane) aminopeptidase antibody, membrane alanyl aminopeptidase antibody, alanyl (membrane) aminopeptidase b antibody, alanyl aminopeptidase, membrane L homeolog antibody, ANPEP antibody, SERPINF2 antibody, GPT antibody, Gpt antibody, Apn2 antibody, Apn1 antibody, SCO2643 antibody, pepN antibody, Shewana3_0897 antibody, Alvin_1024 antibody, Ndas_3413 antibody, Saut_1544 antibody, Fbal_1877 antibody, ML5_3424 antibody, Despr_1988 antibody, Mesop_5365 antibody, Anpep antibody, LOC101744250 antibody, anpepb antibody, anpep.L antibody
- 背景
- CD13 is expressed on the surface of early committed progenitors and mature granulocytes and monocytes (GM-CFU), but not on lymphocytes, platelets or erythrocytes. It is also expressed on endothelial cells, epithelial cells, bone marrow stroma cells, and osteoclasts, as well as a small proportion of LGL lymphocytes. CD13 acts as a receptor for specific strains of RNA viruses and plays an important function in the interaction between human cytomegalovirus (CMV) and its target cells.
- 途径
- Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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