NF-kB p65 抗体 (AA 297-308)
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Quick Overview for NF-kB p65 抗体 (AA 297-308) (ABIN209104)
抗原
See all NF-kB p65 (NFkBP65) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 297-308
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特异性
- Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.
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预测反应
- Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%) Paramecium (83%).
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纯化方法
- Immunoaffinity purified
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免疫原
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A synthetic peptide, RIEEKRKRTYET, corresponding to the NLS of the human NFkB p65 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%), Paramecium (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: ELISA (1:100 - 1:1000), IF (1:200), IHC (1:100), WB (1:1000)
Usage: Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Western Blot: 1:1000. Immunohistochemistry: 1:100. Immunofluorescence microscopy: 1:100. Super shift: 0.5-1 μL/assay. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- RELA / NFKB p65
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背景
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Name/Gene ID: RELA
Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65 -
基因ID
- 5970
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UniProt
- Q04206
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
抗原
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