NFYB 抗体 (N-Term)
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- 抗原 See all NFYB 抗体
- NFYB (Nuclear Transcription Factor Y, beta (NFYB))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NFYB antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 特异性
- NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- 纯化方法
- Ion exchange chromatography
- 免疫原
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Type of Immunogen: Native protein - Top Product
- Discover our top product NFYB Primary Antibody
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- 应用备注
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Approved: ELISA, IP, WB (1:500)
Usage: Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 μL per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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- 抗原
- NFYB (Nuclear Transcription Factor Y, beta (NFYB))
- 别名
- NFYB (NFYB 产品)
- 别名
- CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody
- 背景
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Name/Gene ID: NFYB
Synonyms: NFYB, CBF-A, CBF-B, HAP3, NF-YB - 基因ID
- 4801
- UniProt
- P25208
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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