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ERK1 抗体 (AA 372-406)

Name: ERK1 抗体 (AA 372-406)
SKU: ABIN199418
Availability: OutOfStock

This anti-ERK1 antibody is a 兔多克隆 antibody detecting ERK1 in WB, IP 和 Func. Suitable for 小鼠, 大鼠, 兔和 Hamster.

产品编号 ABIN199418

发货至: 中国

Datasheet as PDF

Quick Overview for ERK1 抗体 (AA 372-406) (ABIN199418)

抗原

See all ERK1 (MAPK3) 抗体

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

适用

小鼠, 大鼠, 兔, Hamster

宿主

兔

克隆类型

多克隆

标记

This ERK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Discover our top product MAPK3 Primary Antibody

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AA 372-406

特异性

Recognizes rat MAPK1/Erk1 at 44kD and MAPK2/Erk2 at 42kD. Species cross-reactivity: Human, mouse, chicken and starfish. Species sequence Homology: Chinese hamster: 35/35, mouse: 34/35, human: 32/35.

预测反应

Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%) Monkey, Bovine, Dog, Bat, Horse, Platypus (97%) Human, Gorilla, Gibbon, Elephant (94%) Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%) Xenopus (84%).

纯化方法

Immunoaffinity purified

免疫原

Synthetic peptide (CGG-PFTFDMELDDLPKERLKELIFQETARFQPGAPEAP) corresponding to the C-terminal 35 amino acids of rat 44kD MAP Kinase 2/ Erk2 (KLH coupled). Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%), Monkey, Bovine, Dog, Bat, Horse, Platypus (97%), Human, Gorilla, Gibbon, Elephant (94%), Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%), Xenopus (84%).

Type of Immunogen: Synthetic peptide - KLH conjugated

亚型

IgG
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 101-200) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibody
MAPK3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-100) antibody
MAPK3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
MAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E5 unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibody
MAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibody
MAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (N-Term) antibody
Verified MAPK3 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr185), (pThr202), (pTyr187), (pTyr204) antibody
MAPK3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibody
MAPK3 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 3C9 unconjugated

应用备注

Approved: Func, IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.1-2 μg/mL detects MAP kinases in RIPA lysates of mouse 3T3/A31 fibroblasts and L6 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MAP Kinase 1/2 (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation Kinase Assay: 4 μg immunoprecipitates active MAP kinases from a lysate of NGF-stimulated (50 ng/mL) PC-12 cells, as demonstrated using the MAPK Immunoprecipitation Kinase Cascade Kit.

说明

Target Species of Antibody: Rat

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 0.1 mM EDTA.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeat freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
抗原

ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

别名

MAPK3 / ERK1

背景

Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

基因ID

5595

UniProt

P27361

途径

MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins