ERK1 抗体 (C-Term)
Quick Overview for ERK1 抗体 (C-Term) (ABIN199417)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Reacts strongly with ERK1 and ERK2. A synthetic peptide, corresponding to a 15 amino acid sequence near the carboxyl terminus (339-353 aa of rat ERK1) was conjugated to carrier protein and used as the immunogen. Reactivity to ERK1 and ERK2 has been confirmed by western blot analysis of human A431 and K562 cells, mouse brain and rat brain. Human, mouse and rat (positive control: human A431 and K562 cells, mouse brain and rat brain cell lysates).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide (a.a. 339-353) corresponding to a C-terminal segment of rat ERK1.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA (0.1 - 1 μg/mL), IHC (5 - 10 μg/mL), IP, WB
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. ELISA: 0.1-1 μg/mL (to coat: 10 μg/mL). Immunoprecipitation: 2-5 μg. Immunohistochemistry: 5-10 μg/mL. Western blotting: ~1:1000. Positive control: Human A431 and K562 cells, mouse brain and rat brain cell lysates). -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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别名
- MAPK3 / ERK1
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背景
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Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3 -
基因ID
- 5595
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UniProt
- P27361
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
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