AKT2 抗体 (Ser474)
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Quick Overview for AKT2 抗体 (Ser474) (ABIN197358)
抗原
See all AKT2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser474
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特异性
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Akt2 antibody AP02678PU detects endogenous levels of total Akt2 protein.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S).
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应用备注
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Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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别名
- AKT2 / PKB beta
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背景
- The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
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基因ID
- 208
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NCBI登录号
- NP_001617
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UniProt
- P31751
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途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
抗原
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