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RAF1 抗体 (Ser259)
This anti-RAF1 antibody is a 兔 多克隆 antibody detecting RAF1 in WB 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
Quick Overview for RAF1 抗体 (Ser259) (ABIN197102)
抗原
See all RAF1 抗体
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
适用
All reactivities for RAF1 抗体
人, 小鼠, 大鼠
宿主
All hosts for RAF1 抗体
兔
克隆类型
All clonalities for RAF1 抗体
多克隆
标记
All conjugates for RAF1 抗体
This RAF1 antibody is un-conjugated
应用范围
All applications for RAF1 抗体
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
RAF1抗体详情
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抗原表位
All epitopes for RAF1 抗体
Ser259
特异性
Raf-1 antibody detects endogenous levels of total Raf-1 protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-SP-T-P).
Alternatives
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使用细节
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应用备注
Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
贮存及处理
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浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
RAF1目标详情
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抗原
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
别名
RAF1
背景
Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
基因ID
5894
NCBI登录号
NP_002871
UniProt
P04049
途径
MAPK Pathway , RTK signaling , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , cAMP Metabolic Process , Stem Cell Maintenance , Hepatitis C , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling , BCR Signaling
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原发性抗体
anti-RAF1 抗体 (Ser259) (ABIN197102)
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