This Osteopontin (1B20) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein. Image: Western Blot of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.
纯化方法
Purified
免疫原
N-terminal, extracellular region of , human Calcium Sensing Receptor.
Western Blot 1:50-1:200, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunoprecipitation 5-10 µg Radioimmunoassay. Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
Liquid with Tris-glycine 150mM NaCl pH 7.5.
注意事项
Repeated freeze/thaw cycles , compromise the integrity of the antiserum.
储存条件
-20 °C
储存方法
Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C.