ELISA: 1/5000. Western blot: 1/500. Immunocytochemistry on Frozen Sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in aqua bidest to a concentration of 1 mg/mL.
浓度
2.0 mg/mL
缓冲液
50 mM Tris, pH 7.4
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized at 2-8 °C and reconstituted at -20 °C.
Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.Synonyms: 180 kDa bullous pemphigoid antigen 2, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, LABD97, LAD-1