Suggested dilutions: Western blotting: 1.100-1.500
限制
仅限研究用
状态
Liquid
缓冲液
0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1 % Sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
-20 °C
储存方法
Store at -20 °C/1 year
有效期
12 months
抗原
RHOA
(Ras Homolog Gene Family, Member A (RHOA))
别名
RhoA
背景
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades.Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility.The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane.It is thought to be important in cell locomotion.Overexpression of this gene is associated with tumor cell proliferation and metastasis.Multiple alternatively spliced variants, encoding the same protein, have been identified.References: Graham D.L.et al.Chem.Biol.9:375-381(2002) Snyder J.T.et al.Nat.Struct.Biol.9:468-475(2002) Longenecker K.et al.Acta Crystallogr.D 59:876-880(2003) Synonyms: RhoA protein, Aplysia ras related homolog 12, ARH 12, ARH12