FAS 抗体
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北京 101111
Quick Overview for FAS 抗体 (ABIN187302)
抗原
See all FAS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for human Fas. Species Reactivity: Human. Other species not tested.
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免疫原
- Mouse L cells stably over-expressing human Fas.
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亚型
- IgG3
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anti-TNF Receptor Superfamily, Member 6 (FAS) antibody
FAS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 150-250) antibodyFAS 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) antibodyFAS 适用: 人 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 4F8H6 unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 87-109), (N-Term) antibodyFAS 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 22-172) antibodyFAS 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 22-169) antibodyFAS 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 21-170) antibodyFAS 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 1-335) antibodyFAS 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1B6 unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 20-119) antibodyFAS 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 5B4 unconjugated
anti-TNF Receptor Superfamily, Member 6 (FAS) (AA 20-119) antibodyFAS 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2G3 unconjugated
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应用备注
- Functional activity: Induces apoptosis, no cross-linking required. Can be used in DISC (death-inducing signaling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. Flow cytometry The optimal dilution for a specific application should be determined by the researcher. Figure 1. Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells. Method: (A) Dose response: Jurkat cells (1x105) were treated with agonistic anti-Fas 2R2 antibody at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry. (B) Time course of apoptosis: Jurkat cells (1x105) were treated with 1 µg/mL anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA. Figure 2. Detection of Fas surface expression by FACS analysis. Method: Normal L cells (open histogram) and cells stably over-expressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunostaining was obtained with an isotype matched IgG control.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 µg monoclonal antibody at 1 mg/mL purified from concentrated hybridoma tissue culture supernatant. Prepared in PBS containing protein stabilizer. Protein A-affinity purified. Purity 95% by SDS-PAGE.
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储存条件
- 4 °C
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- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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别名
- CD95 / FAS
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途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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