Flow Cytometry Immunofluorescence PBMC: Add 10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 min. at 4 °C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Whole Blood: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 min. at RT. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for lysed whole blood and immunofluorescence analysis with a flow cytometer or microscope. The optimal dilution for a specific application should be determined by the researcher.
限制
仅限研究用
缓冲液
Antibody supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for RPE.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders.