FAS 抗体 (FITC)
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北京 101111
Quick Overview for FAS 抗体 (FITC) (ABIN187286)
抗原
See all FAS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Species Reactivity: Human. Others not tested.
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亚型
- IgG1
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应用备注
- Monitoring of activated T cells in peripheral blood, Analysis of NK subsets, Study of B cell activation. Flow cytometry Immunofluorescence PBMC: Add 10 µL of mAb/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 4 °C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Whole Blood: Add 10 µL of mAb/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (RT). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for lysed whole blood and immunofluorescence analysis with a flow cytometer or microscope. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 1.0 mL of FITC-labeled monoclonal antibody in PBS, 0.08% sodium azide and protein stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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别名
- CD95 / FAS
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背景
- Activation of either the 55 kDa tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders.
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途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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