Pituitary Adenylate Cyclase Activating Peptide (AA 17-176) 抗体
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- 抗原
- Pituitary Adenylate Cyclase Activating Peptide
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抗原表位
- AA 17-176
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Immunocytochemistry (ICC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Polyclonal Antibody to Pituitary Adenylate Cyclase Activating Peptide (PACAP)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against PACAP. It has been selected for its ability to recognize PACAP in immunohistochemical staining and western blotting.
- 交叉反应
- 小鼠
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Pituitary Adenylate Cyclase Activating Peptide (PACAP) corresdonding to Ile17~Leu176 with N-terminal His and GST Tag
- 亚型
- IgG
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anti-Pituitary Adenylate Cyclase Activating Peptide (AA 17-176) antibody
适用: 人 ICC, WB, ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pituitary Adenylate Cyclase Activating Peptide (AA 1-175) antibody适用: 小鼠 ICC, WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- Pituitary Adenylate Cyclase Activating Peptide
- 别名
- PACAP antibody, adenylate cyclase activating polypeptide 1 antibody, ADCYAP1 antibody, Adcyap1 antibody
- 背景
- ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide
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