beta-Adrenergic Receptor Kinase (AA 398-640) 抗体
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- 抗原
- beta-Adrenergic Receptor Kinase
- 抗原表位
- AA 398-640
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Polyclonal Antibody to Beta Adrenergic Receptor Kinase (BARK)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against BARK. It has been selected for its ability to recognize BARK in immunohistochemical staining and western blotting.
- 交叉反应
- 小鼠
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Beta Adrenergic Receptor Kinase (BARK) corresdonding to Asp398~Leu640 (Accession # P25098) with N-terminal His and GST Tag
- 亚型
- IgG
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anti-beta-Adrenergic Receptor Kinase (AA 398-640) antibody
适用: 人 ICC, IHC, IP, WB 宿主: 兔 Polyclonal unconjugated
anti-beta-Adrenergic Receptor Kinase (AA 398-640) antibody适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- beta-Adrenergic Receptor Kinase
- 别名
- Beta Adrenergic Receptor Kinase
- 别名
- BARK1 antibody, BETA-ARK1 antibody, GRK2 antibody, G protein-coupled receptor kinase 2 antibody, GRK2 antibody
- 背景
- ADRbK1, B-ARK1, bARK1, Beta-ARK1, GRK2, G-protein coupled receptor kinase 2, Adrenergic Receptor Beta Kinase 1
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