ERK1 抗体 (N-Term)
Quick Overview for ERK1 抗体 (N-Term) (ABIN185399)
抗原
See all ERK1 (MAPK3) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- MAPK3/ERK1
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序列
- GGEPRRTEGV GP
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特异性
- No cross-reactivity expected with ERK2
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence GGEPRRTEGVGP-C, from the N Terminus of the protein sequence according to NP_002737.2, NP_001035145.1, NP_001103361.1.
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 101-200) antibody
MAPK3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibodyMAPK3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 1-100) antibodyMAPK3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibodyMAPK3 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibodyMAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 75-312) antibodyMAPK3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr185), (pThr202), (pTyr187), (pTyr204) antibodyMAPK3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 279-379) antibodyMAPK3 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 3C9 unconjugated
anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pThr202), (pTyr204) antibodyMAPK3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 40 kDa band observed in Human Cerebellum lysates and in lysates of cell lines A431 and HepG2, and approx 42 kDa obseved in Human Frontal Cortex lysates (calculated MW of 40.1 kDa according to NP_001035145.1 and 43.1 kDa according to NP_0
Peptide ELISA: antibody detection limit dilution 1:4000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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别名
- MAPK3
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背景
- MAPK3, ERK1, mitogen-activated protein kinase 3, HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44), HS44KDAP, HUMKER1A, MGC20180, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-re
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基因ID
- 5595
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NCBI登录号
- NP_002737, NP_001035145, NP_001103361
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
抗原
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