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IRAK4 抗体 (N-Term)

This anti-IRAK4 antibody is a 山羊 多克隆 antibody detecting IRAK4 in WB, ELISA, IHC, FACS 和 IF. Suitable for 人.
产品编号 ABIN185338
发货至: 中国

Quick Overview for IRAK4 抗体 (N-Term) (ABIN185338)

抗原

See all IRAK4 抗体
IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

适用

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宿主

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山羊

克隆类型

  • 98
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多克隆

标记

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This IRAK4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    N-Term

    原理

    IRAK4 (N Terminus)

    序列

    NKPITPSTYV RC

    交叉反应

    人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    NKPITPSTYVRC

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 50 kDa band observed in lysates of cell line HeLa and approx 55 kDa in lysates of cell line Jurkat (calculated MW of 51.5 kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentra

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concent

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

    别名

    IRAK4

    背景

    IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1

    基因ID

    51135, 266632

    NCBI登录号

    NP_057207, NP_001107654

    途径

    NF-kappaB Signaling, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
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