IRAK4 抗体 (N-Term)
Quick Overview for IRAK4 抗体 (N-Term) (ABIN185338)
抗原
See all IRAK4 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- IRAK4 (N Terminus)
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序列
- NKPITPSTYV RC
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交叉反应
- 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- NKPITPSTYVRC
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 50 kDa band observed in lysates of cell line HeLa and approx 55 kDa in lysates of cell line Jurkat (calculated MW of 51.5 kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentra
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
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别名
- IRAK4
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背景
- IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1
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基因ID
- 51135, 266632
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NCBI登录号
- NP_057207, NP_001107654
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途径
- NF-kappaB Signaling, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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