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KAP1 抗体 (C-Term)

Name: KAP1 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN184913
Availability: OutOfStock

This anti-KAP1 antibody is a 山羊多克隆 antibody detecting KAP1 in ELISA, IHC, IF 和 FACS. Suitable for 人. This Primary Antibody has been cited in 1 publication.

ABIN184913 (2µg/ml) staining of paraffin embedded Human Breast. (KAP1 抗体 (C-Term))

IHC

ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer). (KAP1 抗体 (C-Term))

ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast. (KAP1 抗体 (C-Term))

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (KAP1 抗体 (C-Term))

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (KAP1 抗体 (C-Term))

ABIN184913 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (KAP1 抗体 (C-Term))

FACS

6 images

(1 reference)

产品编号 ABIN184913

发货至: 中国

Quick Overview for KAP1 抗体 (C-Term) (ABIN184913)

抗原

See all KAP1 (TRIM28) 抗体

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

适用

人

宿主

山羊

克隆类型

多克隆

标记

This KAP1 antibody is un-conjugated

应用范围

ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified

抗原表位
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C-Term

原理

KAP1 / TRIM28

序列

SSQELSGGPG DGP

交叉反应

人, 小鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

亚型

IgG
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 4E6 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibody
TRIM28 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 适用: 人 WB, IF, IP, ICC, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibody
TRIM28 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibody
TRIM28 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 1-835) antibody
TRIM28 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

应用备注

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:2000.

说明

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).
抗原

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

别名

TRIM28

背景

TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein

基因ID

10155

NCBI登录号

NP_005753

途径

Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus