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KAP1 抗体 (C-Term)

There is 1 publication for this product available. The 山羊 多克隆 anti-KAP1 antibody is suitable to detect KAP1 in samples from 人. It has been validated for IHC, ELISA, IF 和 FACS.
产品编号 ABIN184913
发货至: 中国
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Quick Overview for KAP1 抗体 (C-Term) (ABIN184913)

抗原

See all KAP1 (TRIM28) 抗体
KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This KAP1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
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    C-Term

    原理

    KAP1 / TRIM28

    序列

    SSQELSGGPG DGP

    交叉反应

    人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:2000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).

  • 抗原

    KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

    别名

    TRIM28

    背景

    TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein

    基因ID

    10155

    NCBI登录号

    NP_005753

    途径

    Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
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