GRB2 抗体 (C-Term)
Quick Overview for GRB2 抗体 (C-Term) (ABIN184670)
抗原
See all GRB2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- GRB2
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序列
- PRNYVTPVNR NV
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特异性
- This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.
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亚型
- IgG
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anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody
GRB2 适用: 人, 小鼠, 大鼠, 犬, 小鸡 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibodyKO Validated GRB2 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 89-118) antibodyGRB2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 156-217) antibodyGRB2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人, 小鼠, 大鼠, Cow, Hamster WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (N-Term) antibodyGRB2 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 6E1 unconjugated Recombinant Antibody
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 1-217) antibodyGRB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4C6-H6 unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 198-217) antibodyGRB2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 26 kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2 kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
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别名
- GRB2
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背景
- GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096,
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基因ID
- 2885, 14784, 81504
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NCBI登录号
- NP_002077, NP_987102
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途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
抗原
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