E Tag 抗体 (HRP)
WB, ELISA
宿主: 兔
Polyclonal
HRP
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- 抗原 See all E Tag products
- E Tag
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This E Tag antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA
- 序列
- GAPVPYPDPL EPR
- 特异性
- Anti-E-tag [HRP] Polyclonal Antibody recognizes E tagged fusion proteins.
- 产品特性
- Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH
- 亚型
- IgG
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anti-E Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 11H12B3 unconjugated
anti-E Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- 浓度
- 0.5 mg/mL
- 缓冲液
- lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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- 抗原
- E Tag
- Abstract
- E Tag 产品
- 物质类
- Tag
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