Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL Western blot: 0. 1-1. 0 µg/mL Other applications: user-optimized
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
浓度
0.5 mg/mL
缓冲液
lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Anania, Bustos, Lill, Kirkpatrick, Coscoy: "A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway." in: International journal of proteomics, Vol. 2013, pp. 857918, (2013) (PubMed).
Uribe, Almaraz Barrera, Robles-Flores, Mendoza Hernández, González-Robles, Hernández-Rivas, Guillen, Vargas: "A functional study of nucleocytoplasmic transport signals of the EhNCABP166 protein from Entamoeba histolytica." in: Parasitology, Vol. 139, Issue 13, pp. 1697-710, (2012) (PubMed).