PKD-PKCmu (pSer738) 抗体
适用: 人, 小鼠, 大鼠
WB, IF
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- PKD-PKCmu
- 抗原表位
- pSer738
- 适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Detects endogenous levels of PKD/PKCµ, only when phosphorylated at serine 738.
- 产品特性
- Molecular Weight: Predicted Band Size 115 kDa, Observed Band Size 115 kDa
- 纯化方法
- The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- Synthesized phosphopeptide derived from human PKD/PKCµ, around pSer738 (E-K-SP-F-R).
- 亚型
- IgG
-
-
- 应用备注
-
WB: 1:500-1:1,000,
IF: 1:100-1:200 - 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
-
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing. - 储存条件
- -20 °C
- 储存方法
- Store at -20 °C for 1 year.
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- 抗原
- PKD-PKCmu
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