IGFBP7 抗体 (Biotin)
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- 抗原 See all IGFBP7 抗体
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IGFBP7 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects IGFBP7
- 纯化方法
- Affinity purified
- 免疫原
- Highly pure (> 98 %) recombinant human IGFBP7
- Top Product
- Discover our top product IGFBP7 Primary Antibody
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- 应用备注
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ELISA: Direct: To detect hIGF-BP7 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7. Western Blot: To detect hIGF-BP7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIGF-BP7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- 有效期
- 6 months
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- 抗原
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
- 别名
- IGFBP7 (IGFBP7 产品)
- 别名
- zgc:85888 antibody, AGM antibody, FSTL2 antibody, IBP-7 antibody, IGFBP-7 antibody, IGFBP-7v antibody, IGFBPRP1 antibody, MAC25 antibody, PSF antibody, RAMSVPS antibody, TAF antibody, Fstl2 antibody, Mac25 antibody, insulin like growth factor binding protein 7 antibody, insulin-like growth factor binding protein 7 antibody, IGFBP7 antibody, igfbp7 antibody, Igfbp7 antibody
- 背景
- IGF-BPs form high affinity complexes with both IGF-I and IGF-II and act to control of the distribution, function and activity of IGFs in various cell tissues and body fluids. There are seven named IGF-BPs. IGFBP7 plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. IGFBP7 is expressed in a wide range of normal human tissues and it usually shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin.Synonyms: IBP-7, IBP7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, Insulin-like growth factor-binding protein 7, MAC25, PGI2-stimulating factor, PSF, Prostacyclin-stimulating factor, TAF, Tumor-derived adhesion factor
- 基因ID
- 3490
- NCBI登录号
- NP_001544
- UniProt
- Q16270
- 途径
- Growth Factor Binding
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