Glu-alpha-Tubulin (C-Term) 抗体
适用: 人, 小鼠, 大鼠, Eukaryotes, 斑马鱼
WB, ELISA, IHC, ICC
宿主: 小鼠
Monoclonal
1D5
unconjugated
(9 references)-
- 抗原
- Glu-alpha-Tubulin
- 抗原表位
- C-Term
- 适用
- 人, 小鼠, 大鼠, Eukaryotes, 斑马鱼
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).
- 交叉反应 （详细）
- No cross reaction to tyrosinated tubulin.
- 纯化方法
- purified IgG. Azide was added before lyophilization.
- 免疫原
- Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.
- 克隆位点
- 1D5
- 亚型
- IgG1
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- 应用备注
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WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200
IHC: 1 : 200 - 说明
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WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS, 0.02% sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).
: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." in: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).
: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." in: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).
: "Two catalytic domains are required for protein deacetylation." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).
: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." in: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).
: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." in: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).
: "Monocilia on chicken embryonic endocardium in low shear stress areas." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).
: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." in: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).
: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." in: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).
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: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).
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- 抗原
- Glu-alpha-Tubulin
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