GABRA1 抗体 (AA 28-43)
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- 抗原 See all GABRA1 抗体
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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抗原表位
- AA 28-43
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适用
- 大鼠, 小鼠
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宿主
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豚鼠
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克隆类型
- 多克隆
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标记
- This GABRA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Specific for GABA-A receptor alpha1.
- 纯化方法
- antiserum
- 免疫原
- Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Top Product
- Discover our top product GABRA1 Primary Antibody
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- 应用备注
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WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000
IHC: 1 : 500 - 说明
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS
- 注意事项
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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- 抗原
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
- 别名
- GABA-A Receptor Alpha1 (GABRA1 产品)
- 别名
- ECA4 antibody, EJM antibody, EJM5 antibody, zgc:153566 antibody, Gabra-1 antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antibody, gamma-aminobutyric acid receptor subunit alpha-1 antibody, GABRA1 antibody, Gabra1 antibody, gabra1 antibody, LOC100653509 antibody
- 背景
- Synonyms: GABA(A) R alpha1
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