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GABRA1 抗体 (AA 28-43)

GABRA1 适用: 大鼠, 小鼠 WB, IHC, ICC 宿主: 小鼠 Monoclonal 53D3 unconjugated
产品编号 ABIN1742455
发货至: 中国
  • 抗原 See all GABRA1 抗体
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    抗原表位
    • 21
    • 20
    • 20
    • 20
    • 15
    • 15
    • 15
    • 14
    • 11
    • 11
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-43
    适用
    • 130
    • 106
    • 86
    • 14
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    大鼠, 小鼠
    宿主
    • 101
    • 73
    • 2
    小鼠
    克隆类型
    • 101
    • 75
    单克隆
    标记
    • 53
    • 12
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This GABRA1 antibody is un-conjugated
    应用范围
    • 166
    • 80
    • 75
    • 70
    • 33
    • 28
    • 26
    • 26
    • 17
    • 12
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    特异性
    Specific for GABA-A receptor alpha1.
    纯化方法
    purified IgM. Azide was added before lyophilization.
    免疫原
    Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
    克隆位点
    53D3
    亚型
    IgM
    Top Product
    Discover our top product GABRA1 Primary Antibody
  • 应用备注
    WB: 1 : 1000 up to 1 : 2000 (AP staining)
    IP: not tested yet
    ICC: 1 : 500
    IHC: 1 : 500
    说明

    ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    缓冲液
    PBS, 0.02% sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    储存条件
    -20 °C
    储存方法
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).

  • 抗原
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    别名
    GABA-A Receptor Alpha1 (GABRA1 产品)
    别名
    ECA4 antibody, EJM antibody, EJM5 antibody, zgc:153566 antibody, Gabra-1 antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antibody, gamma-aminobutyric acid receptor subunit alpha-1 antibody, GABRA1 antibody, Gabra1 antibody, gabra1 antibody, LOC100653509 antibody
    背景
    Synonyms: GABA(A) R alpha1
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